Test and optimize mRNA candidates with our high-throughput in vitro screening solutions
In Vitro Screening
Our In Vitro Screening platform provides research teams with reliable, data-driven insights into mRNA construct performance. Designed to support the earliest stages of mRNA therapeutic development, our platform can help you evaluate key mRNA performance parameters with speed and precision.
Some of the assays available through our In Vitro Screening platform:
High-Throughput In Vitro Expression Testing
Validate structural integrity of translated mRNA constructs in mammalian cell lines of interest, and quantify expression in high throughput. Optimize mRNA sequence designs based on expression results.
Cell-Free mRNA Thermal Stability Testing
Evaluate cell-free mRNA thermal stability and melting temperature; use mRNA structural stability data to inform mRNA sequence design strategies.
Cell Viability Assays
Evaluate the dose-dependent impact of mRNA transfection on cellular health using standard cell viability assays (e.g., Alamar Blue, XTT).
Application-Specific Assays
Customize your mRNA testing with specialized assays tailored to your application (e.g., enzyme activity assays), to gain deeper insights into construct performance in specific contexts.
Why Choose Our Platform?
Expert Guidance
Work with a team of experienced scientists dedicated to supporting your research goals.
Actionable Insights
Obtain clear data to guide mRNA construct selection and optimization.
Tailored Solutions
Choose assays that align with your specific project needs.
Streamlined Development
Save time and resources by identifying and resolving mRNA performance issues early in the development process.
Partner with Us
Our In Vitro Screening platform is designed to streamline your mRNA research and provide reliable data to move your work forward. Whether you need to evaluate mRNA stability, expression, functionality, or safety, we can provide the scientific knowhow and deliverables that you need to advance your project.
Contact us to learn how our In Vitro Screening platform can support your next mRNA breakthrough.
Examples
mRNA thermal stability curves for three different N1-methylpseudouridine-modified FLuc mRNA designs, as measured by differential scanning fluorimetry. mRNA melting temperature is denoted by the ▼ icon.
Quantification of protein expression in HeLa cells transfected with FLuc mRNA variant #2 (closed circles) or #3 (open circles), and harboring unmodified bases, N1-methylpseudouridine base modification, 5-methoxyuridine base modification, or N1-methylpseudouridine and 5-methylcytidine base modification.
Quantification of kinetic enzyme activity [enzyme X] in SH-SY5Y cells transfected with vehicle control, mRNA-encoded positive control, or mRNA-encoded enzyme X variants #A, #B or #C.
Get in touch.
Email us at help@messenger.bio or fill out the form below, and we will get back to you within 48 hours.